Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] NB7539 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemGoat anti-Mouse IgG (H+L) Secondary Antibody [HRP]
CompanyNovus Biologicals, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberNB7539
ImmunogenThis Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] was developed against mouse IgG-heavy and light chain
Concentration1.0 mg/ml
Conjugate/TagHRP
FormatPhosphate Buffered Saline (PBS) containing 0.2% BSA
Quantity1 ml
ApplicationsWestern Blot, ELISA, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
ReactivityMouse
HostGoat
TargetIgG (H+L)
IsotypeIgG
ClonalityPolyclonal
PurityImmunogen affinity purified
Brand/TrademarkNovus Biologicals, a Bio-Techne Brand
Antibody TypeSecondary Antibodies
Research AreaAdaptive Immunity, Immunology
StorageStore at 4C. Do not freeze
Target SpeciesMouse (Ms)

Specifications/Features

Available conjugates: FITC, HRP

Specificity: By immunoelectrophoresis and ELISA this Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins

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Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: http://www.novusbio.com

(1) Early-life stress triggers long-lasting organismal resilience and longevity via tetraspaninbioRxiv : the preprint server for biologyDecember 17, 2023Jiang WI, De Belly H, Wang B, Wong A, Kim M, Oh F, DeGeorge J, Huang X, Guang S, Weiner OD, et al.each with Tris-buffered saline with 0.1% Tween 20, goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] (NB7539, Novus) added at a dilution of 1:5000 as the secondary antibody. Fluorescein assay Stage-synchronized

(2) Transgenic mouse models support a protective role of type I IFN response in SARS-CoV-2 infection-related lung immunopathology and neuroinvasionCell reportsNovember 28, 2023Chauhan NR, Kundu S, Bal R, Chattopadhyay D, Sahu R, Mehto S, Yadav R, Krishna S, Jena KK, Satapathy S, et al.

(3) Intranasal VLP-RBD vaccine adjuvanted with BECC470 confers immunity against Delta SARS-CoV-2 challenge in K18-hACE2-miceVaccineJuly 31, 2023Lee KS, Rader NA, Miller-Stump OA, Cooper M, Wong TY, Shahrier Amin M, Barbier M, Bevere JR, Ernst RK, Heath Damron F.were washed 4x before adding 100 µL secondary antibody (goat anti-mouse IgG HRP; Novus Biologicals NB7539) (diluted 1:2000) in 1% nonfat milk-PBS-Tween20 to all wells and shaking for an additional 1 hr at room

(4) Human cells experience a Zn2+ pulse in early G1Cell ReportsJune 17, 2023Ananya Rakshit, Samuel E. Holtzen, Maria N. Lo, Kylie A. Conway, Amy E. Palmer,Santa Cruz Biotechnology,Cat# Sc-5266; RRID:AB_627308 Goat anti-mouse (H + L) IgG secondary,Novus Biotechnology,Cat# NB7539 Goat anti-Rabbit (H + L) IgG secondary,Novus Biotechnology,Cat# NB7160 ,, "Chemicals, peptides, and

(5) Characterization of an Aedes ADP-Ribosylation Protein Domain and Role of Post-Translational Modification during Chikungunya Virus InfectionPathogens (Basel, Switzerland)May 16, 2023Kumar R, Mehta D, Nayak D, Sunil S.antibody were incubated with anti-mice IgG HRP antibody (Novus Biologicals, Colorado, USA, Cat. no. NB7539, 1:6000 dilution), washed with PBST, and then visualized using the Bio-Rad ChemiDoc MP System (Bio-Rad

(6) Secreted Soluble Factors from Tumor-Activated Mesenchymal Stromal Cells Confer Platinum Chemoresistance to Ovarian Cancer CellsInternational journal of molecular sciencesApril 23, 2023Koren Carmi Y, Khamaisi H, Adawi R, Noyman E, Gopas J, Mahajna J.instructions. Secondary antibodies, HRP-linked anti-rabbit (#7074, Cell Signaling Technology), and anti-mouse (NB7539 Novus, Centennial, CO, USA) were used, as per the manufacturers’ instructions. Chemiluminescence was

(7) Bendless is essential for PINK1-Park mediated Mitofusin degradation under mitochondrial stress caused by loss of LRPPRCPLoS geneticsApril 1, 2023Cheramangalam RN, Anand T, Pandey P, Balasubramanian D, Varghese R, Singhal N, Jaiswal SN, Jaiswal M.A32733,1:500 IF Anti-Rabbit HRP,Novus Biologicals- NB7160,1:5000 WB Anti-Mouse HRP,Novus Biologicals- NB7539,1:5000 WB Antibodies used in this study. IF- Immunofluorescence WB- Western Blot

(8) In Vivo Efficacy and Safety Evaluations of Therapeutic Splicing Correction Using U1 snRNA in the Mouse RetinaCellsMarch 21, 2023Swirski S, May O, Ahlers M, Wissinger B, Greschner M, Jüschke C, Neidhardt J.anti-GAPDH antibody (Merck, Chemicon, MAB374) was used. A peroxidase-conjugated goat anti-mouse antibody (NB7539, Novus) was used as the secondary antibody, followed by ECL detection. Relative protein levels were

(9) Production and characterization of cell-penetrating recombinant botulinum neurotoxin type ASTAR protocolsMarch 17, 2023Wei X, Hu J, Jiang L, Li L, Liu J."Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (1:1000 diluted in 0.2% BSA-PBS)",Novus,NB7539 ,, Bacterial and virus strains,Bacterial and virus strains,Bacterial and virus strains ,, Sf9 cells,Thermo Fisher

(10) Influence of Canonical and Non-Canonical IFNLR1 Isoform Expression on Interferon Lambda SignalingVirusesFebruary 25, 2023Evans JG, Novotny LA, Meissner EG.°C in 1% milk/TBST. After washing, blots were incubated with goat anti-mouse IgG conjugated to HRP (NB7539, Novus Biologicals, Bio-Techne, Minneapolis, MN, USA) for 1 h at room temperature and then developed

(11) A TARP Syndrome Phenotype Is Associated with a Novel Splicing Variant in RBM10GenesNovember 18, 2022Owczarek-Lipska M, Markus F, Bültmann E, Korenke GC, Neidhardt J.incubated with horse radish peroxidase (HRP)-linked secondary antibodies (Novus Biological, NBP2-30348H and NB7539, both 1:5000) at room temperature for 1 h and with enhanced chemiluminescence solution (ECL, Thermo

(12) Impact of CHIKV Replication on the Global Proteome of Aedes albopictus CellsProteomesNovember 10, 2022Kumar R, Mehta D, Chaudhary S, Nayak D, Sunil S.membrane was then incubated with anti-mice HRP antibody (1:6000 dilution) (Novus Biologicals, Cat. NB7539, Centennial, CO, USA) in PBST + 2.5% BSA for 1 h at RT, followed by washing with PBST for 3 × 10 min

(13) Microenvironmentally optimized 3D-printed TGFβ-functionalized scaffolds facilitate endogenous cartilage regeneration in sheepActa biomaterialiaSeptember 15, 2022Yang Z, Cao F, Li H, He S, Zhao T, Deng H, Li J, Sun Z, Hao C, Xu J, et al.

(14) Integrated bioactive scaffold with aptamer-targeted stem cell recruitment and growth factor-induced pro-differentiation effects for anisotropic meniscal regenerationBioengineering & translational medicineSeptember 1, 2022Li H, Zhao T, Cao F, Deng H, He S, Li J, Liu S, Yang Z, Yuan Z, Guo Q.overnight at 4°C, followed by incubation with a goat anti‐mouse IgG secondary antibody (1:200; Cat# NB7539; Novus) for 1 h at room temperature. The diaminobenzidine (DAB) substrate system was used for color

(15) AAV-mediated delivery of an anti-BACE1 VHH alleviates pathology in an Alzheimer's disease modelEMBO molecular medicineApril 7, 2022Marino M, Zhou L, Rincon MY, Callaerts-Vegh Z, Verhaert J, Wahis J, Creemers E, Yshii L, Wierda K, Saito T, et al.with HRP‐conjugated goat‐anti‐mouse IgG antibody diluted in blocking buffer (1:10,000 dilution, Novus, NB7539) for 1 h at room temperature. After incubation, the membranes were washed three times in washing buffer

(16) PI3K-AKT Pathway Modulation by Thymoquinone Limits Tumor Growth and Glycolytic Metabolism in Colorectal CancerInternational journal of molecular sciencesFebruary 19, 2022Karim S, Burzangi AS, Ahmad A, Siddiqui NA, Ibrahim IM, Sharma P, Abualsunun WA, Gabr GA.antibodies used were either goat anti-rabbit IgG-HRP (NB7160, Novus Biologicals) or goat anti-mouse IgG-HRP (NB7539, Novus Biologicals). 4.8. Glucose Uptake/Lactate Production, ATP, and NADPH Measurement Glucose uptake

(17) 2-Hydroxyestradiol Overcomes Mesenchymal Stem Cells-Mediated Platinum Chemoresistance in Ovarian Cancer Cells in an ERK-Independent FashionMolecules (Basel, Switzerland)January 26, 2022Khamaisi H, Mahmoud H, Mahajna J.instructions. Secondary antibodies, HRP-linked anti-rabbit (#7074, Cell Signaling Technology) and anti-mouse (NB7539 Novus, Centennial, CO, USA) were used according to the manufacturers’ instructions. Chemiluminescence

(18) Simvastatin mitigates streptozotocin-induced type 1 diabetes in mice through downregulation of ADAM10 and ADAM17Life sciencesJanuary 15, 2022Abdel-Bakky MS, Alqasoumi A, Altowayan WM, Amin E, Darwish MA.

(19) Autosomal dominant optic atrophy: A novel treatment for OPA1 splice defects using U1 snRNA adaptionMolecular therapy. Nucleic acidsDecember 3, 2021Jüschke C, Klopstock T, Catarino CB, Owczarek-Lipska M, Wissinger B, Neidhardt J.anti-GAPDH antibody (Merck, Chemicon, MAB374) was used. A peroxidase-conjugated goat anti-mouse antibody (NB7539, Novus) was used as secondary antibody, followed by enhanced chemiluminescence (ECL) detection. Relative

(20) A novel missense variant in the EML1 gene associated with bilateral ribbon-like subcortical heterotopia leads to ciliary defectsJournal of human geneticsDecember 1, 2021Markus F, Kannengießer A, Näder P, Atigbire P, Scholten A, Vössing C, Bültmann E, Korenke GC, Owczarek-Lipska M, Neidhardt J.Afterwards, the membrane was incubated with horse radish peroxidase coated secondary antibodies (1:5,000, NB7539, NB7160, Novus Biologicals, Centennial, Colorado, USA) at room temperature for 1 h and afterwards with

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Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP]

Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP]

Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP]

Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP]

Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] from Novus Biologicals, a Bio-Techne Brand

Specifications/Features

Novus Biologicals, a Bio-Techne Brand

Citations (42)

Reviews

Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] from Novus Biologicals, a Bio-Techne Brand

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